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GATE 2017-2018 :: GATE Biotechnology

GATE Biotechnology - Practice Test Paper 1
GATE Biotechnology - Practice Test Paper 2
  1. A callus of 5 g dry weight was inoculated on semi-solid medium for growth. The dry weight of the callus was found to increase by 1.5 fold after 10 days of inoculation. The growth index of the culture is _________
    2. A.
    0.3
    B.
    0.5
    C.
    0.7
    D.
    0.9

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  2. A chemostat is operated at a dilution rate of 0.6 h-1. At steady state, the biomass concentration in the exit stream was found to be 30 g l-1. The biomass productivity (g l-1h-1) after 3h of steady state operation will be ___________
    3. A.
    3
    B.
    9
    C.
    18
    D.
    27

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  3. A batch bioreactor is to be scaled up from 10 to 10,000 liters. The diameter of the large bioreactor is 10 times that of the small bioreactor. The agitator speed in the small bioreactor is 450 rpm. Determine the agitator speed (rpm) of the large bioreactor with same impeller tip speed as that of the small bioreactor. _________
    4. A.
    45
    B.
    40
    C.
    50
    D.
    55

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  4. Calculate the percentage sequence identity for the pairwise alignment given below. _______ 
    H E L L O “ 
    Y E L L O W
    5. A.
    33.6 to 33.8
    B.
    25.9 to 26.1
    C.
    54.7 to 54.9
    D.
    66.5 to 66.7

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  5. In a batch culture, the specific rate of substrate utilization is 0.25 g (g cell mass)-1 h-1 and specific rate of product formation is 0.215 g (g cell mass)-1 h-1. Calculate the yield of product from the substrate(Yp/s). ___________
    6. A.
    0.81 to 0.91
    B.
    0.91 to 1.01
    C.
    3.45 to 3.55
    D.
    2.65 to 2.75

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  6. Match the commercial microbial sources in Group I with the products in Group II.
    Group I                       Group II
    P. Corynebacteriumlilium 1. 2,3-Butane di-ol
    Q. Klebsiellaoxytoca   2. Poly-β-hydroxybutyric acid
    R. Aspergillusniger       3. Glutamic acid
    S. Alcaligeneseutrophus  4. Citric acid
    7. A.
    P-3,Q-1,R-2,S-4
    B.
    P-3,Q-1,R-4,S-2
    C.
    P-1,Q-3,R-2,S-4
    D.
    P-1,Q-3,R-4,S-2

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  7. Match the entries in the Group I with the elution conditions in Group II.
    Group I                                  Group II
    P. Ion-exchange chromatography  1. Isocratic solvent
    Q. Hydrophobic column chromatography  2. Ampholytes
    R. Gel filtration chromatography  3. Increasing gradient of salt
    S. Chromatofocusing  4. Decreasing gradient of polarity
    8. A.
    P-4,Q-1,R-2,S-3
    B.
    P-4,Q-3,R-1,S-2
    C.
    P-3,Q-4,R-1,S-2
    D.
    P-3,Q-4,R-2,S-1

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  8. Determine the correctness or otherwise of the following Assertion (a) and Reason (r).
    Assertion: Immobilization of plant cells can enhance secondary metabolite production during bioreactor cultivation. 
    Reason: Immobilization protects the plant cells from shear forces in the bioreactor.
    9. A.
    Both (a) and (r) are true and (r) is the correct reason for (a).
    B.
    Both (a) and (r) are true but (r) is not the correct reason for (a).
    C.
    (a) is true but (r) is false.
    D.
    (a) is false but (r) is true.

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  9. Match the cell structures in Group I with the organismsin Group II.
    Group I                        Group II
    P. Endospores           1. Methanobacterium
    Q. Bipolar flagella     2. Treponema
    R. Pseudomurine in cell wall  3. Spirillum
    S. Periplasmic flagella   4. Clostridium
    10. A.
    P-4, Q-3, R-1, S-2
    B.
    P-4, Q-3, R-2, S-1
    C.
    P-3, Q-4, R-1, S-2
    D.
    P-4, Q-1, R-3, S-2

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  10. Match the antibioticsin Group I with the targets in Group II.
    Group I                        Group II
    P. Sulfonamide    1. Peptidoglycan synthesis
    Q. Quinolones      2. Peptide chain elongation
    R. Erythromycin   3. Folic acid biosynthesis
    S. Cephalosporin  4. Topoisomerase
    11. A.
    P-3, Q-4, R-1, S-2
    B.
    P-2, Q-4, R-3, S-1
    C.
    P-4, Q-1, R-2, S-3
    D.
    P-3, Q-4, R-2, S-1

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