GATE 2017-2018 :: GATE Biotechnology
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A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII,BamHIandEcoRI individually. Restriction digestion yielded following fragments. Plasmid + HindIII→ 1200bp and3800bpPlasmid + BamHI→5000bpPlasmid + EcoRI→ 2500bpThe number of sites for EcoRI,BamHIandHindIIIpresent on this plasmid are
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The total number of fragments generated by the complete and sequential cleavage of the polypeptide given below by Trypsin followed by CNBr is _________ Phe-Trp-Met-Gly-Ala-Lys-Leu-Pro-Met-Asp-Gly-Arg-Cys-Ala-Gln
- In a genetic study, 80 people were found to have alleles for polydactyly. Only 36 of them were polydactylous. What is the extent of penetrance percentage? ________
- One percent of the cars manufactured by a company are defective. What is the probability (upto four decimals) that more than two cars are defective, if 100 cars are produced? __________
- The maximum cell concentration (g l-1) expected in a bioreactor with initial cell concentration of 1.75 g l-1 and an initial glucose concentration of 125 g l-1 is (Yx/s = 0.6 g cell/g substrate) ________
- A fed batch culture was operated with intermittent addition of glucose solution at a flow rate of 200 ml h-1. The values of Ks, μm and D are 0.3 g l-1, 0.4 h-1 and 0.1 h-1, respectively. Determine the concentration of growth limiting substrate (gl-1) in the reactor at quasi-steady state. ____________
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A solution was prepared by dissolving 100 mg of protein X in 100 ml of water. Molecular weight of protein X is 15,000 Da; Avogadro's number = 6.022 * 1023.Calculate the molarity(μM) of the resulting solution.
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The binding efficiency of three different receptorsR1, R2 and R3 were tested against a ligand using equilibrium dialysis, with a constant concentration of receptor and varying concentrations of ligand. The Scatchard plot of receptor titration with different concentration of ligand is given below (ris moles of bound ligand per moles of receptor and c is concentration of free ligand)
The number of ligand binding sites present on receptors R1 and R3, respectively are -
The binding efficiency of three different receptorsR1, R2 and R3 were tested against a ligand using equilibrium dialysis, with a constant concentration of receptor and varying concentrations of ligand. The Scatchard plot of receptor titration with different concentration of ligand is given below (ris moles of bound ligand per moles of receptor and c is concentration of free ligand)Which one of the receptors has the highestaffinity for the ligand?
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A DNA fragment of 5000bp needs to be isolated from E.coli (genome size 4*103kb) genomic library.The minimum number of independent recombinant clones required to represent this fragment in genomic library are
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