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GATE 2017-2018 :: GATE Biotechnology

  1. A protein is phosphorylated at a serine residue. A phosphomimic mutant of the protein can be generated by substituting that serine with
  2. A.
    glycine
    B.
    alanine
    C.
    aspartate
    D.
    threonine

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  3. A truncated polypeptide is synthesized due to a nonsense mutation. Where would you introduce another mutation to obtain a full-length polypeptide?
  4. A.
    Ribosomal protein gene
    B.
    Transfer RNA gene
    C.
    DNA repair gene
    D.
    Ribosomal RNA gene

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  5. Protein-DNA interactions in vivo can be studied by
  6. A.
    gel shift assay
    B.
    Southern hybridization
    C.
    chromatin immunoprecipitation assay
    D.
    fluorescence in situ hybridization assay

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  7. The direction of shell coiling in the snail Limnaea peregra is a classic example of
  8. A.
    chromosomal inheritance
    B.
    extra-chromosomal inheritance
    C.
    chromosomal translocation
    D.
    homologous recombination

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  9. During photorespiration under low CO2 and high O2 levels, O2 reacts with ribulose 1,5- bisphosphate to yield
  10. A.
    one molecule each of 3-phosphoglycerate and 2-phosphoglycolate
    B.
    two molecules of 3-phosphoglycerate
    C.
    two molecules of 2-phosphoglycolate
    D.
    one molecule each of 3-phosphoglycerate and glyoxylate

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  11. Which one of the following is NOT a protoplast fusion inducing agent?
  12. A.
    Inactivated Sendai virus
    B.
    Ca2+ at alkaline pH
    C.
    Polyethylene glycol
    D.
    Colchicine

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  13. The activity of an enzyme is expressed in International Units (IU). However, the S.I. unit for enzyme activity is Katal. One Katal is
  14. A.
    1.66 * 104 IU
    B.
    60 IU
    C.
    6 * 107 IU
    D.
    106 IU

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  15. Identify the statement that is NOT applicable to an enzyme catalyzed reaction.
  16. A.
    Enzyme catalysis involves propinquity effects
    B.
    The binding of substrate to the active site causes a strain in the substrate
    C.
    Enzymes do not accelerate the rate of reverse reaction
    D.
    Enzyme catalysis involves acid-base chemistry

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  17. An example of a derived protein structure database is
  18. A.
    Pfam
    B.
    SCOP
    C.
    GEO
    D.
    Prosite

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  19. An example of a program for constructing a phylogenetic tree is
  20. A.
    phylip
    B.
    phrap
    C.
    prodom
    D.
    PHDsec

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