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GATE 2017-2018 :: GATE Biotechnology

  1. Match the entries in Group I with the methods of sterilization in Group II.
    Group I                 Group II
    P. Serum             1. Autoclave
    Q. Luria broth    2. Membrane filtration
    R. Polypropylene tubes 3. UV irradiation
    S. Biological safety cabinets 4. Gamma irradiation
                                   5. Dry heat
  2. A.
    P-5, Q-3, R-1, S-4
    B.
    P-1, Q-4, R-5, S-3
    C.
    P-2, Q-1, R-4, S-3
    D.
    P-4, Q-1, R-3, S-5

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  3. Match the high energy compounds in Group I with the biosynthetic pathways for the molecules in Group II.
    Group I       Group II
    P. GTP         1. Fatty acid
    Q. UTP        2. Phospholipid
    R. CTP         3. Protein
    S. Acyl coenzyme A  4. Peptidoglycan
  4. A.
    P-3, Q-2, R-4, S-1
    B.
    P-2, Q-4, R-3, S-1
    C.
    P-4, Q-3, R-1, S-2
    D.
    P-3, Q-4, R-2, S-1

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  5. Match the vitamins in Group I with the processes/reactions in Group II.
    Group I                            Group II
    P. Pantothenic acid    1 . Electron transport
    Q. Vitamin B2             2. Transfer of 1-C units
    R. Vitamin B6             3. Decarboxylation
    S. Folic acid               4 . Fatty acid metabolism
                                       5. Hydrolysis
  6. A.
    P-5, Q-2, R-4, S-1
    B.
    P-4, Q-1, R-3, S-2
    C.
    P-4, Q-2, R-1, S-3
    D.
    P-2, Q-1, R-3, S-5

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  7. Consider the data set 14, 18, 14, 14, 10, 29, 33, 31, 25. If you add 20 to each of the values, then
  8. A.
    both mean and variance change
    B.
    both mean and variance are unchanged
    C.
    the mean is unchanged, variance changes
    D.
    the mean changes, the variance is unchanged

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  9. An enzymatic reaction is described by the following rate expression. 
    v = (vms)/(km + s + s2/ks)
    Which one of the following curves represents this expression?
  10. A.
    .

    B.
    .

    C.
    .

    D.
    .

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  11. A bacterial culture (200 l containing 1.8 * 109 cells) was treated with an antibiotic Z (50 μg per ml) for 4 h at 37ËšC. After this treatment, the culture was divided into two equal aliquots. 
    Set A: 100 μl was plated on Luria agar. 
    Set B: 100 μl was centrifuged, the cell pellet washed and plated on Luria agar. 
    After incubating these two plates for 24 h at 37ËšC, Set A plate showed no colonies, whereas the Set B plate showed 0.9 * 109 cells. This experiment showed that the antibiotic Z is
  12. A.
    bacteriostatic
    B.
    bacteriocidal
    C.
    bacteriolytic
    D.
    apoptotic

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  13. In a muscle, the extracellular and intracellular concentrations of Na+ are 150 mM and 12 mM, and those of K+ are 2.7 mM and 140 mM, respectively. Assume that the temperature is 25oC and that the membrane potential is -60 mV, with the interior more negatively charged than the exterior. (R = 8.314 J mol-1 K-1; F = 96.45 kJ mol-1 V-1
    The free energy change for the transport of three Na+ out of the cell is
  14. A.
    +1.5 kJ/mol
    B.
    +17.4 kJ/mol
    C.
    +18.9 kJ/mol
    D.
    +36.3 kJ/mol

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  15. In a muscle, the extracellular and intracellular concentrations of Na+ are 150 mM and 12 mM, and those of K+ are 2.7 mM and 140 mM, respectively. Assume that the temperature is 25oC and that the membrane potential is -60 mV, with the interior more negatively charged than the exterior. (R = 8.314 J mol-1 K-1; F = 96.45 kJ mol-1 V-1
    The free energy change for the transport of two K+ into the cell is
  16. A.
    +8.0 kJ/mol
    B.
    +11.6 kJ/mol
    C.
    +19.6 kJ/mol
    D.
    +31.2 kJ/mol

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  17. The purification data for an enzyme is given below:

    The fold purification for each step is
  18. A.
    P-0.1, Q- 0.66, R-0.84, S- 1.26
    B.
    P-1.0, Q-0.52, R-0.67, S-0.8
    C.
    P-1, Q-6.6, R-8.4, S-12.6
    D.
    P-100, Q-66, R-84, S-12

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  19. The purification data for an enzyme is given below:

    The yield (%) for each step is
  20. A.
    P-10, Q-7.2, R-4.5, S-2.0
    B.
    P-34, Q-24, R-15, S-1
    C.
    P-3.4, Q-2.4, R-1.5, S-0.1
    D.
    P-100, Q-71, R-44, S-29

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